Phylogenetic Analysis of Lactobacillus plantarum and Related Species Using Partial Housekeeping Genes
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Abstract:
Phylogenetic trees of L. plantarum strains were constructed to explore the usage of the 16S rRNA gene, pheS and pyrG gene sequences for distinguishing Lactobacillus plantarum from its related species.. The phylogenetic trees of ten L. plantarum strains isolated from sourdough, yoghurt, and pickles were constructed using partial sequences of housekeeping genes, pheS and pyrG, as molecular markers. These trees were compared with other phylogenetic trees constructed using the 16S rRNA gene sequence of L. plantarum combined with the corresponding gene sequences of closely related strains already published in GenBank. The results showed that L. plantarum and its related species could be distinguished by analyzing the 16S rRNA gene sequence; however, pheS and pyrG sequences could be used for this classification. Additionally, it was found that sequential use of pheS and pyrG could clearly distinguish strains from reference strains. Thus, a combination of genes (pheS-pyrG) could be used as an auxiliary tool along with 16S rRNA gene analysis for rapid classification and identification of L. plantarum and related species.
