Structure Analysis of Uronic Acid-containing Polysaccharides in Viscera of Four Types of Fish
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Abstract:
Viscus is one of the major by-products of fish processing. To fully utilize this biological resource, polysaccharides from the viscera of Ctenopharyngodon idellus, Cyprinus carpio, Larimichthys crocea, and Trichiurus lepturus were analyzed. Polysaccharides were isolated by double enzyme hydrolysis (trypsin and papain), followed by hydrolyzing with 1.3 M trifluoroacetic acid at 105 ℃ for 3 h. Disaccharides from acid hydrolysates were analyzed using HPLC-MS/MS after 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. By comparing the retention times and mass spectra of the resultant polysaccharides with those of the references, the uronic acid-containing polysaccharides (UACPs) in the fish viscera were identified. The results showed that hyaluronic acid (HA), chondroitin sulfate (CS), heparin (HP), and an unknown UACP-containing repeated disaccharide with hexuronic acid linked to hexose were present in the viscera of C. idellus. CS, HP, and small quantities of HA were detected in the viscera of C. carpio. CS was found to be the major UACP in the viscera of L. crocea and T. lepturus. The results revealed the UACPs in the viscera of four types of fish and provided a scientific basis for exploring and utilizing these resources.
