Study on the Constituents and Antioxidant Activity of Gold Tea Alcohol Extract
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Abstract:
The in vitro anti-oxidant activity and the constituents of gold tea ethanol extract were investigated. The structure of the crude extract was examined using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after ethanol extraction as well as petroleum ether and ethyl acetate extraction. The anti-oxidant activity was then analyzed in vitro. The results showed that gold tea ethanol extract was composed of chlorogenic acid, p-coumaroylquinic acid, caffeic acid, rutin, hyperin, kaempferol-3-O-rutinoside, ferulic acid, eriodictyol-O-hexoside, and luteoloside. The ethanol extract was found to exhibit a high antioxidant capacity in vitro. At the same concentration, the DPPH radical scavenging activity of the ethanol extract was higher than that of Vitamin C (Vc). The extract also had a high hydroxyl radical scavenging ability, which increased in a dose-dependent manner. In addition, the extract had the ability to scavenge superoxide anions, and this ability reached 89.80% at a concentration of 250 μg/mL. When the ABTS+ radical scavenging activity was measured, the ethanol extract was found to have higher activity than Vc, when the concentration was 50~100 μg/mL; when the concentration was 100~250 μg/mL, the scavenging activity of the two were comparable. At the same concentration, the reducing capacity of the extract was slightly lower than that of Vc.
