Vasodilator and Anti-inflammatory Effects of Quercetin and Hyperin from Blueberry Leaves Identified by HPLC-MS in Umbilical Vein Endothelial Cells

Abstract:

TNF-α induction was used to establish an in vitro inflammatory injury model in human umbilical vein endothelial cells (HUVECs). The effects of quercetin and hyperin from blueberry leaves on angiotensin I converting enzyme (ACE) level and the content of inflammatory factors (monocyte chemoattractant protein-1, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1) in HUVECs was investigated. Soluble ACE and inflammatory factor protein content in supernatants was detected by ELISA to explore the vasodilator and anti-inflammatory function of quercetin and hyperin in endothelial cells. The results showed that the content of ACE and inflammatory factor soluble proteins was significantly increased in TNF-α-induced cells. The protein expression of ACE and inflammatory factors in HUVECs were significantly decreased (p<0.05) when the cells were treated with quercetin or hyperin, showing that both exhibited anti-inflammatory and vasodilator effects.