Glycosylation of CD8+ T Lymphocytes from C57BL/6 Mice Recognized by Agrocybe aegerita Lectins
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Abstract:
Two novel lectins, AAL (Agrocybe aegerita lectin) and AAL2 (Agrocybe aegerita lectin 2), were employed to detect the glycosylation of CD8+ T lymphocytes from C57BL/6 mouse spleen and inguinal lymph nodes. Biotinylated AAL and AAL2, combined with flow cytometry, were used to analyze the glycosylation of CD8+ T lymphocytes and cytokine expression. The proportion (%) of AAL+CD8+ and AAL2+CD8+ T lymphocytes in spleen was found to be 6.72±3.00 and 12.18±5.28, respectively. The proportion (%) of AAL+CD8+ and AAL2+CD8+ T lymphocytes in lymph nodes was 12.18±5.28 and 10.15±3.46, respectively. Moreover, the frequency of perforin+ cells in spleen AAL2+CD8+ T cells was lower than that in AAL2-CD8+ T cells (p<0.05), and the frequency of perforin+ cells in lymph node AAL+CD8+ T cells was higher than that in AAL-CD8+ T cells (p<0.05). This study provides new data on the glycosylation of spleen and inguinal lymph node CD8+ T cells and the application of two new lectins, AAL and AAL2.
