Identification of the Main Nitrogen-containing Compounds in Ctenopharyngodon Idellus by HPLC-Q-TOF-MS
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Abstract:
The main nitrogenous compounds in methanol extracts of Ctenopharyngodon idellus were analyzed and identified using high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). Both positive and negative ionization modes were used to confirm the related chemical compounds and their characteristic fragment ions according to the accurate molecular mass information of the excimer ion peaks and the fragment ions. Using the ChemSpider database, 33 nitrogenous compounds in Ctenopharyngodon idellus were ultimately determined, and the data for the main fragments of each component were analyzed. These 33 nitrogenous compounds included 11 amino acids (eight types of α-amino acids), 12 amines, eight amide compounds, and two heterocyclic nitrogenous compounds. The results showed that the structures of monomer compounds in fresh aquatic products and raw materials could be elucidated directly using the HPLC-Q-TOF-MS technique and fragmentation analysis by mass spectrometry, which can improve the efficiency of analysis and identification of chemical components in fresh raw material. This study shows that these techniques are conducive for the identification and analysis of active components and newly generated compounds during the storage of fresh raw materials.
