Safety Evaluation and Anti-anemia Effect of Hairtail Protein Ferrous-chelating Peptide in Mice
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Abstract:
A rat model of iron deficiency anemia was established by feeding rats an iron-deficient diet, and the anti-anemia effect and safety of hairtail protein ferrous-chelating peptide were studied by feeding the animals with hairtail protein ferrous-chelating peptide through gavage, and these effects were evaluated using the acute toxicity test, Ames test, mouse bone marrow micronucleus test, sperm abnormality test and chromosome aberration test in mammalian cells in vitro. The results showed that hairtail protein ferrous-chelating peptide increased the hemoglobin (Hb) content and the number of red blood cells (RBC) and other peripheral parameters, and significantly improved the symptoms of anemia in rats. The median lethal dose (LD50) was 7454.80 mg/kg, indicating actual non-toxicity. TA97, TA98, TA100, and TA102, four standard strains in Ames test, were all negative in the Ames test of the mice. Meanwhile, compared with the negative control group, the results of the mouse bone marrow micronucleus test, sperm abnormality test, and chromosome aberration test in mammalian cells in vitro showed no significant difference, suggesting that the three results were also all negative. Therefore, we concluded that hairtail protein ferrous-chelating peptide has an anti-anemia effect and good food safety, and can be further developed and utilized as a food additive.
