Effects of HIU-aided Pretreatment of Crude Protein in Fish Skin: Separation and Structure
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Abstract:
Due to the larger consumption of energy, solvent, and pretreatment period during the conventional separation of crude protein in fish skin, a method of HIU-aided pretreatment of fish skin was provided. In order to reveal the effects of different ultrasonic pulses ways on fish skin and the structure of fish collagen fiber, five following parameters were investigated: total content of proteins removed, loss of collagen during the HIU pretreatment, structure of collagen fiber after the HIU pretreatment, subsequent extraction yield of collagen and SDS-PAGE patterns of collagen. After pretreatment by the way of HIU3 s on 2 s off for 60 min, the content of protein in fish skin removed was 37.37 mg/g without significant loss of collagen (0.63 mg/g). SEM figures showed that, the loose of fish skin and the sufficient extension of collagen fiber were convenient for the subsequent collagen extraction after pretreatment by the way of HIU3 s on 2 s off. FT-IR spectra obtained for fish collagen fiber confirmed the integrated conformation of fish skin collagen. Furthermore, collagen with structural integrity well maintained yielded the highest.
