Microflora Analysis and Preliminary Identification of Dominant Spoilage Bacteria in Refrigerated Cololabis saira
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
A deeper understanding of the dominant spoilage bacteria during storage will help develop methods to inhibit target bacteria, thus extending the shelf life of Cololabis saira. Changes in the bacterial phase of the bacteria causing spoilage of Cololabis saira stored at 4°C were investigated, the dominant spoilage bacteria were isolated and identified, and their spoilage potential was verified. The results showed that the shelf life of refrigerated Cololabis saira was six days. A total of 13 strains that belonged to seven genera, were isolated during refrigerated storage, including seven gram-positive and six gram-negative bacteria. Furthermore, according to the change in the proportion of each strain in the bacterial phase and their effects on the total volatile basic nitrogen (TVB-N) value of sterilized fish juice, Strains 13 (Enterobacter sp.), 12 (Shewanella sp.), 7, and 9 (Acinetobacter sp.) were identified. These bacteria had different influences on the TVB-N, trimethylamine (TMA) and 2-2-thiobarbituric acid (TBA) content of Cololabis saira. Strain 12 (Shewanella sp.) accelerated TMA generation, Strain 13 (Enterobacter sp.) showed significant influence on TVB-N value, Strain 7 (Acinetobacter sp.) significantly increased TBA content, while Strain 9 (Acinetobacter sp.) affected all three indicators. The results demonstrated that the four identified strains showed strong spoilage potential for Cololabis saira during refrigerated storage.
