Screening and Tracing of Anti-alzheimer Related Bioactive Constituents from Marine Fungi
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Abstract:
Active constituents having anti-Alzheimer’s effects were screened and traced in 44 marine fungal strains. Acetylcholinesterase (AChE) inhibitory and antioxidant activities were assessed using the 5,5-dithio-bis-2-nitrobenzoic acid method and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging method, respectively. Bioautography and specific color-developing reagents were applied to investigate the diversity of bioactive substances and their structural types, respectively. HPLC-Q-TOF-ESI-HRMS was used to characterize representative fungal bioactive metabolites. The fermentation extracts of 32 strains in different culture media showed remarkable dual bioactivity against AChE and DPPH. These dual-bioactive strains originated from different fungal taxa. Bioautography revealed their diverse bioactive constituents. Chemical colorization further suggested that their active components were nitrogenated compounds (alkaloids), phenols, and other types of natural products. Based on HPLC-Q-TOF-ESI-HRMS analysis, a metabolite with molecular formula C16H13NO2 of a strain exhibited relatively strong inhibition effect against AChE. The present study revealed that marine fungi were an important source of diverse anti-Alzheimer’s active substances, and provided a foundation for future studies involving the isolation and elucidation of bioactive compounds.
