Protective Effect of Plastein Reaction-modified Casein Hydrolysates on H2O2-induced Injury in Human Umbilical Vein Endothelial Cells
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Abstract:
Casein was hydrolyzed by neutrase (0.8 L), and then the hydrolyzed casein (HC) was modified with phenylalanine (PHE), histidine (HIS), and proline (PRO) through plastein reaction. In vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH?) radical-scavenging abilities, hydroxyl radical-scavenging abilities, and reducing powers of the corresponding products (HCPHE, HCHIS, and HCPRO) were significantly higher than those of HC by 42.81%, 45.83%, and 30.51%, respectively (p<0.05). The modified products (HCPHE, HCHIS, and HCPRO) were incubated with human umbilical vein endothelial cells (HUVECs) for 24 h, and the protective effect of the modified products was evaluated after the HUVECs were injured using 300 μmol/L H2O2. Cell viability was measured using the CCK-8 method. The results showed that the cell viability of the groups treated with a high dose of HCPHE, HCHIS, or HCPRO was significantly higher than that of the group treated with HC by 7.86%, 5.21%, or 10.52% (p<0.05), respectively, and there was no significant difference between the high dose group and the group treated with tea polyphenols (TP) (p>0.05); furthermore, the relationship was dose-dependent. HCPHE, HCHIS, and HCPRO effectively decreased the leakage of lactate dehydrogenase (LDH), malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity, compared with the HC group at the same concentration. Furthermore, HCPHE, HCHIS, and HCPRO significantly enhanced the activities of glutathione reductase (GSH-Rd) and catalase (CAT), while the change in the content of glutathione (GSH) in the cells did not significantly change. The study showed that HCPHE, HCHIS, and HCPRO had a good protective effect on H2O2-induced injury in HUVECs, with the protective effect being better than that of HC.
