Anticancer and Immunoregulation Activities of a Polysaccharide from Russula vinosa
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Abstract:
Human cervical squamous cell carcinoma (SiHa) cells were chosen to study the in vitro antitumor activities of a Russula vinosa polysaccharide (HAP-I), and the results were confirmed using the scratch assay. Furthermore, an in vitro proliferation test with spleen cells from Kunming mice, neutral red uptake experiments, and analyses of the secretion of nitric oxide (NO) and interleukin-6 (IL-6) by RAW264.7 macrophages were conducted to study the anticancer and immunomodulatory activities of HAP-I. The results showed that in a concentration range of 125~2000 μg/mL, the inhibitory effect of HAP-I on SiHa was increased with increasing concentration and was dose-dependent to some extent. The scratch assay also demonstrated the inhibitory effect of HAP-I on the proliferation of SiHa. HAP-I could promote the proliferation of spleen cells from Kunming mice over a concentration range of 10~500 μg/mL, and this proliferation was dramatically promoted by the synergistic effect of concanavalin A (ConA) and lipopolysaccharide (LPS). The combination of HAP-I (at a concentration of 50 μg/mL or 100 μg/mL) and LPS had a significant effect on the proliferation; the optimal synergistic effect of HAP-I with ConA was found at 100 μg/mL. HAP-1 not only enhanced the ability of macrophages for neutral red uptake, but also increased the secretion of NO and IL-6 by macrophages, indicating that HAP-I has strong immunological activities.
