Identification of Microbial Flora in Cow Shelter and Raw Milk by Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF-MS)
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Dairy farms of different scales in the surrounding area of Harbin were selected as the study objects, and the number of microorganisms and flora composition in cow shelters and the microbial species present in raw milk were determined and analyzed in both spring and summer. The aim of this study was to explore the effects of the breeding environment on the quality and safety of raw milk. The Andersen six-stage sampler was employed to collect microorganism samples in the air, and the samples were cultured at 37 ℃ for 48 h, followed by counting, isolation, and purification. The isolated cultures were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Results showed significant differences in microbial content in the same cow shelter between spring and summer (p<0.05). No significant differences were found in the microbial content among different cow shelters in the season during which the shelter was enclosed. In the season during which the cow shelter was open, there were no significant differences in microbial content between cow shelters A and C (p>0.05). However, both of these shelters were significantly different from cow shelter C in terms of microbial content (p<0.05). There were 116 strains of bacteria identified in the air of cow shelters, including 32.76% Staphylococcus spp. and 45.69% Bacillus spp.; there were 56 strains of bacteria identified in the raw milk, including 41.07% lactic acid bacteria, 30.06% Enterobacter spp., and 12.50% Staphylococcus spp. The homology between microorganisms in the air of cow shelters and those in the from raw milk was not significant.
