Screening of Probiotic Bifidobacterium Strains from Infants and Study of Their Intestinal Colonization Ability
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Abstract:
Bifidobacterium strains with potential probiotic properties were screened from infant feces and their colonization ability in the intestine was explored, to provide potential probiotic strains for the development of Bifidobacterium products. De Man, Rogosa, and Sharpe (MRS) medium was used to isolate and purify the samples, and detection of fructose-6-phosphate phosphoketolase (F6PPK) and analysis of 16S rDNA sequences were performed on the strains. The probiotic properties of the strains, including tolerance to simulated gastrointestinal fluids and bile salts, inhibition of foodborne pathogenic bacteria (Escherichia coli, Salmonella spp., Listeria monocytogenes, etc.,) and adhesion capacity to HT-29 cells were determined. The screened Bifidobacterium strains with potential probiotic properties were used in animal experiments to measure their intestinal colonization ability. After isolation, 27 Bifidobacterium strains were identified from seven Bifidobacterium species using molecular biology: Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium pseudocatenulatum, Bifidobacterium infantis, Bifidobacterium animalis, and Bifidobacterium adolescentis. In vitro experiments showed that B. longum A9, B. breve A4, B. bifidum B6, B. longum C6, B. adolescentis F8, and B. infantis H6 had stronger potential probiotic properties. The animal experiments showed that B. infantis H6 and B. longum C6 had stronger gut colonization ability. The results demonstrate that B. infantis H6 and B. longum C6 have potential as good probiotic Bifidobacterium strains that can be applied to the development of Bifidobacterium products.
