Hepatoprotective Effects of Bioactive Compounds from Rape Bee Pollen on L-02 Cells Injured by CCl4
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Quercetin-3-O-β-D-glucosyl-(2→l)-β-glucoside (QMP), kaempferol-3,4'-di-O-β-D-glucoside (KMG), and kaempferol-3-O- β-D-glucosyl-(2→l)-β-D-glucoside (KMP) were isolated and purified from rape bee pollen. A model of CCl4-induced liver injury was established via in vitro cultivation of L-02 cells, and the protective effects of QMP, KMG, and KMP on L-02 cells with CCl4-induced injury were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), and lactic dehydrogenase (LDH) were used as the characteristic indicators to study the hepatoprotective effect in terms of oxidative stress. The relative cell survival rate of QMP-treated cells (200 μg/mL) was 2.64 and 2.66 times as much as that of the model group, respectively, suggesting that QMP can effectively prevent CCl4-induced liver injury in L-02 cells, but the hepatoprotective effects of KMG and KMP were not significant. QMP, kaempferol, and quercetin could significantly reduce intracellular MDA levels; the MDA levels of 300 μM dose groups were 0.65, 0.60, 0.59 and 0.52 times as much as that of the model group, respectively; the corresponding LDH leakage rates were reduced by 23.82%, 27.69%, 30.11%, and 32.77%, respectively; the SOD contents were increased by 1.45, 1.61, 1.58, and 1.65, respectively. The results suggest that QMP possesses significant in vitro antioxidant activity and a hepatoprotective effect, the antioxidant activity is one of the mechanisms underlying its hepatoprotective effect. This study provides a basis for developing drugs to treat diseases associated with liver injury.
