Effect of Collagen Hydrolysate on the Properties of Dialdehyde Starch-gelatin Films
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Abstract:
The effect of tilapia skin collagen hydrolysate (TSCH) on the physicochemical properties of dialdehyde starch (DAS) cross-linked gelatin films was investigated, and the biocompatibility of these films with the human skin keratinocyte (HaCat) cell was also studied. When the TSCH content in the DAS-gelatin films was increased from 0 to 16%, the tensile strength (TS) of films decreased from 55.00 MPa to 24.85 MPa, while the elongation at break (EAB) showed a downward trend after the initial increase with increasing amount of added TSCH. The solubility of DAS cross-linked gelatin films in water at 30 ℃ was 33.10%, and at TSCH concentration higher than four percent, it increased to 100%. However, TSCH from films was released into water after 240 min irrespective of the amount of added TSCH. The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the addition of TSCH in the DAS cross-linked gelatin film could hinder the interaction between the protein molecules of gelatin. On the other hand, when HaCat cell was cultured on the surface of DAS cross-linked gelatin films, the cell activity of HaCat cells was 11.39%. When the DAS cross-linked gelatin film contained 8% TSCH, the HaCat cell activity reached up to 108.2%. Based on the above results, the DAS-gelatin films containing TSCH have potential applications in the cosmetic industry.
