Effect of REG1 Deletion with SNF1 Overexpression on the Fermentation of Industrial Baker’s Yeast in Lean Dough
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Abstract:
The effects of REG1 (regulatory subunits of protein phosphatase type 1 PP1) deletion with protein kinase gene SNF1 overexpression on the maltose metabolism and lean dough fermentation by industrial baker’s yeast were investigated by analysis of the MAL mRNA expression, maltase and maltose permease activities, maltose metabolism and leavening ability in lean dough. The basic growth characteristics of REG1 deletion with SNF1 overexpression strain BYKPS-R, the parental strain BY14α, and the REG1 deletion strain BYK-R were also studied. REG1 deletion with SNF1 overexpression had significant effects on increasing MAL gene expression and enzyme activity, and relieving glucose repression, thereby enhancing the maltose utilization efficiency (increase of 18.59% and 4.40% compared with the strains BY14α and BYK-R, respectively, at the point of glucose exhaustion), and lean dough leavening ability (12.51% and 3.22% increases compared with the strains BY14α and BYK-R, respectively). On the background of REG1 deletion, overexpression of SNF1 further improved maltose metabolism and leavening ability of industrial baker’s yeast in lean dough. Moreover, overexpression of SNF1 did not affect growth in REG1 deleted yeast. This strain has potential for application. This study lays a foundation for the breeding of fast-fermenting baker’s yeast.
