Study of Quorum Sensing and Biofilm Formation of Hafnia alvei Separated from Turbot
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Abstract:
One quorum-sensing (QS) strain separated from a spoiled turbot sample was used as the study object and determined as Hafnia alvei (Ha-01) according to physiological and biochemical tests and 16S rRNA analysis. The QS compounds [N-acyl-homoserine lactones (AHLs)] were detected by parallel-streak method using Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136. Additionally, the plate-diffusion method was used to study the kinetics of AHL secretion and the amount of biofilm produced in different growth phases, and exogenous signaling molecules were added to study the relationship between AHLs and biofilm formation. The results indicated that strain Ha-01 produced QS phenomena, and the number of AHLs produced by Ha-01 during cultivation exhibited a downward trend after the initial increase, reaching the maximum at 16 h. The amount of biofilm formed was positively correlated with incubation time and reached a maximum at 72 h, followed by gradual stabilization. The addition of exogenous AHL strands could promote the production of biofilm. These results indicated that strain Ha-01 was capable of producing QS phenomena and regulating biofilm formation.
