Determination of Eight Biogenic Amines in Soy Sauce by Using QuEChERS and Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry
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Abstract:
An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous determination of eight biogenic amines in soybean sauce. 1, 7-Heptanediamine was added to the samples and used as the internal standard. Samples were diluted in water and pH values were adjusted using ammonia water. The sample solution was then extracted by acetonitrile using liquid-liquid extraction and salting out methods. The extract was subsequently purified by using Quick Easy Cheap Effective Rugged Safe (QuEChERS). After the acidity of the extract was adjusted and the solution was concentrated and reconstituted, all samples were separated and measured by using UPLC-MS/MS and quantitatively AND analyzed using an internal standard method. Under the optimized conditions, the calibration curves of eight analytes showed a good linearity over a concentration range of 5.0~500 μg/L, with coefficients of determination (R2) greater than 0.998. The limits of detection (LODs) and the limits of quantitation (LOQs) of the method were in the range of 30~60 μg/kg and 120~250 μg/kg, respectively. The spike and recovery experiments at two levels of concentration in soybean sauce samples (n = 6) were performed. The average recoveries were found to be between 83.7% and 110%, with relative standard deviation (RSD) values of 3.8~11.5%. The detection results on the actual soybean sauce samples revealed that the content of tyramine and phenylethylamine were the highest in the samples. The developed method is accurate, sensitive, rapid, and suitable for the simultaneous determination of eight biogenic amines in soybean sauce.
