Acute Toxicity of T-2 Toxin in Shrimp at Different Growth Stages and Comparison of Corresponding Analytical Methods
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Abstract:
The acute toxicity of T-2 toxin on Litopenaeus vannamei at different growth periods and corresponding analytical methods were compared. Additionally, the optimal period to use shrimps as a biomarker for evaluation of the toxicity of T-2 toxin was determined, and a complementary method for evaluation of the acute toxicity of T-2 toxin on shrimps was established. Acute toxicity tests were performed on L. vannamei at different growth stages. The Spearman-Karber and probability unit methods were used to calculate the lethal dose 50% (LD50) values for T-2 toxin in L. vannamei at different growth stages. The obtained LD50 values were compared by u-tests. The LD50 values for T-2 toxin in shrimp eggs, juvenile shrimps, and adult shrimps were 2.33, 1.79, and 3.34 mg/kg of body weight, respectively, and no significant differences were observed between the LD50 values obtained using the two methods. These findings suggested that T-2 toxin exhibited the lowest toxic effect on adult shrimps and the highest toxic effect on juvenile shrimps. Thus, juvenile L. vannamei were more suitable for biomarker analysis when evaluating the acute toxicity of T-2 toxin, and a relatively accurate LD50 value could be obtained using a method combining the Spearman-Karber method and the probability unit method.
