Comparison of the Antioxidant Activities of Ethanol Extracts from Shuidouchi Samples Fermented using Different Vessels
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Abstract:
The antioxidant activities of Shuidouchi samples fermented in different vessels were determined in this study. First, the physicochemical properties of Shuidouchi samples fermented in different vessels were compared, and glass vessel-fermented Shuidouchi (GVFS) showed lower temperature and acidity and higher moisture contents and total bacteria counts than plastic vessel-fermented Shuidouchi (PVFS) and ceramic vessel-fermented Shuidouchi (CVFS). The active isoflavone contents in Shuidouchi samples fermented in different vessels were measured, and the daidzein and genistein contents in GVFS were higher than those in PVFS and CVFS. The in vitro antioxidant experiments showed that the 2,2-diphenyl-1-picrylhydrazyl (DPPH?) free radical and hydroxyl (?OH) radical scavenging capacities of GVFS were also higher than those of PVFS and CVFS. Animal experiments showed that GVFS increased the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and decreased the levels of nitric oxide (NO) and malondialdehyde (MDA) in serum and liver of mice with D-galactose induced oxidative stress, and these effects of GVFS were better than those of PVFS and CVFS. Reverse transcription polymerase chain reaction (RT-PCR) showed that GVFS upregulated the expression of nNOS, eNOS, Mn-SOD, Gu/Zn-SOD, and CAT mRNA and downregulated iNOS expression in the liver of mice subjected to oxidative stress, indicating that these expression levels were close to those in normal mice. Based on these results, GVFS had higher antioxidant activity than PVFS and CVFS, and a glass vessel is suitable for Shuidouchi production.
