Expression of Bile Salt Hydrolase Genes in Lactobacillus plantarum KLDS1.0386
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The expression levels of four key genes encoding bile salt hydrolase in Lactobacillus plantarum KLDS1.0386 were investigated at the transcriptional level during the different growth phases. Different concentrations of taurodeoxycholate and glycodeoxycholate were used as substrates, 16s rRNA gene was used as the internal standard, and real-time quantitative polymerase chain reaction (PCR) method was used to detect the variations in the expression of the bile salt hydrolase genes (bsh1, bsh2, bsh3, and bsh4) in the presence of taurodeoxycholate and glycodeoxycholate at different concentrations. The results showed that the amplification efficiencies of the reference gene and target genes were in a range of 90~105%, indicating that the expression levels of the target genes were well reflected. The real-time quantitative PCR results showed that the expression levels of four bile salt hydrolase genes increased significantly when the incubation time was prolonged. bsh1, bsh2, bsh3, and bsh4 were expressed in two types of bile salts. The expression levels were upregulated by bile salts at different concentrations (1%, 2%, and 3%), and the increment exhibited an extremely significant upward trend with increasing bile salt concentrations. Additionally, the expression level of each gene was different in the presence of two different bile salts at the same concentration. The gene expression level was generally higher in the presence of glycodeoxycholate (GDC) than that in the presence of taurodeoxycholate (TDC).
