Protective Effect of the Polyphenols from Osmanthus fragrans Flowers on TNF-α-induced Inflammation in HUVECs
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Abstract:
An in vitro human umbilical vein endothelial cell (HUVEC) model of inflammation induced by tumor necrosis factor (TNF)-α was established to study the effects of the purified fractions 1, 2, and 3 obtained from the ethanol extracts of Osmanthus fragrans flowers on cell viability (MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay), reactive oxygen species (ROS) content, and the activity of superoxide dismutase (SOD) and xanthine oxidase (XOD) in the inflammatory cells. The inflammatory reaction was triggered by TNF-α (10 μg/L) in HUVECs, and then the inflammatory cells were treated with fractions 1, 2, and 3 at concentrations of 6000, 3000, 300, and 30 μg/L. The protective effects of the concentrations and compositions of fractions 1, 2, and 3 on inflammatory cells were evaluated. The results showed that treatment with fractions 2 and 3 at concentrations ranging from 300–3000 μg/L and fraction 1 at a concentration of 3000 μg/L could significantly enhance HUVEC cell viability and SOD activity (p < 0.05), while significantly suppressing XOD activity and reducing ROS content (p < 0.05). The abovementioned treatment exhibited strong anti-inflammatory effects.
