Reduction of Bovine β-lactoglobulin Allergenicity via Bifidobacterium longum BBMN68 Modulated-dendritic Cells in Mice
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Abstract:
To clarify the mechanism of probiotics on alleviating food allergy symptoms, bovine β-lactoglobulin (BLG) was used as the allergen to construct a food-allergy mouse model. BALB/c mice were sensitized by oral administration of Bifidobacterium longum BBMN68 (BBMN68). Levels of cytokines and immunoglobulins in the serum and cell culture supernatant were determined by enzyme-linked immunosorbent assay (ELISA), and the changes in the number of CD4+CD25+Foxp3+Treg cells and different subsets of dendritic cells (DCs) were evaluated by flow cytometry. The results showed that BBMN68 alleviated the T-helper 1 (Th1)/T-helper 2 (Th2) imbalance, and reduced hypersensitivity reactions in BLG mice. Compared with the allergy group, the administration of BBMN68 significantly enhanced the expression of CD103 (p < 0.05) and reduced the expression of CD86 and MHC-II (p< 0.05) of DCs in Peyer’s patches (PP). The numbers of CD4+CD25+Foxp3+Treg cells in the lymphocytes from PP, mesenteric lymph node, and spleen were increased by 41.91%, 71.16%, and 61.25%, respectively, compared with those in BLG mice. These DCs modulated with BBMN68 and the CD4+T cells from BLG mice were isolated and cultured together. The results showed an increased proportion of Foxp3+Treg cells and an elevated secretion of regulatory cytokines TGF-β and IL-10 (p < 0.05). These data demonstrated that the mechanism of BBMN68 for reducing BLG allergenicity was associated with the regulation of DC functions and promotion of the DC-mediated immune suppression.
