Study on Natural Borneol-Potentiated Curcumin Inhibiting A375 Melanoma Cell Growth
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In the article, MTT assay was applied to investigate the effect of curcumin alone and a separate natural borneol with different concentrations on cell viability of A375 melanoma cell and analysed the natural borneol combined with curcumin inhibit melanoma A375 cell proliferation by flow cytometry. The experiment concluded that curcumin could significantly inhibit the A375 cell proliferation. However, natural borneol was not showed obvious inhibitory effect emphasized on A375 cell proliferation. Melanoma A375 cell was pretreated with 40 μg/mL natural borneol 3 h and combined with 20 μmol/L curcumin 72 h, the cells survival rate reached a minimum of 10.93%, which was decreased by 18.27% compared to 20 μmol/L curcumin alone. Further investigation on the action mechanisms by flow cytometric analysis showed that SubG1 peaks and G2/M phase ratios notably increased when A375 cells were dealt with natural borneol association with curcumin. Natural borneol (40 μg/mL) and curcumin (20 μmol/L) combined effects of melanoma cells A375, which SubG1 and G2/M respectively increase to 16.8% and 40.1% compared with the content of the control group 2.0% and 16.4%, which revealed the inhibition of natural borneol combined with curcumin on A375 cells was related with apoptosis and G2/M cycle arrest to inhibit A375 cell proliferation.
