Colorimetric Aptasensor for the Determination of Chloramphenicol in Fish and Goose Meat
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
A novel colorimetric aptasensor was developed for rapid identification of chloramphenicol (CAP) in fish and goose samples. The assay is based on a highly selective aptamer binding to its targets followed by a dual-amplification effect from large amounts of palladium oxide (PdO) nanoparticle labeled EnVision (EV). This method could detect ultra-trace amounts of CAP specifically, sensitively, and rapidly. EV has approximately 100 horseradish peroxidase (HRP) molecules and can effectively catalyze the substrate 3,3’,5,5’-tetramethylbenzidine (TMB) for color development. In addition, PdO can catalyze TMB to produce a blue-green color. Thus, a dual-amplification effect can be achieved by using the proposed method. The amount of CAP in samples can be calculated by measuring the change in the absorbance of TMB. The results indicate that, under optimal conditions, this method displays relatively high sensitivity and a good linear correlation with CAP concentration, within the range of 0.02~150 ng/mL. The lower detection limit was identified as 0.01 ng/mL. In addition, this assay was successfully used to analyze CAP concentrations in fish and goose samples, and the results were consistent with those obtained by the conventional ELISA method.
