Preliminary Analysis, Separation, and Purification of the Chemical Components of Minnan Preserved Radish Produced in Different Years
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Abstract:
High performance liquid chromatography-diode array detection (HPLC-DAD) was used to establish the HPLC fingerprints. The similarity evaluation system for traditional Chinese medicine (TCM) chromatographic fingerprint (version A) was applied to analyze and evaluate the similarities of the HPLC fingerprints. The components of five-year preserved radish were separated and purified by using SephadexLH-20 resin, positive phase silica gel column chromatography, and reversed phase C18 (RP-C18) silica gel column chromatography. The structures were identified using thin layer chromatography (TLC), ultraviolet (UV) detection, electrospray ionization mass spectrometry (ESI-MS), and nuclear magnetic resonance spectroscopy (NMR). The liquid chromatograms of the methanol extracts of Minnan preserved radish produced in different years and those of fresh radish were obtained, and the results showed that the similarities between the chemical components of the Minnan preserved radish produced in different years and those of fresh radish were less than 40%. Additionally, two compounds–pinoresinol and linoleic acid methyl ester–were isolated and purified from five-year preserved radish, and both were first found in preserved radish, which provided a basis for the edible and therapeutic value.
