New Method Based on Animal Genetic Information for Identification and Detection of Edible Oil
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Abstract:
The detection limit and detection conditions of edible oil combined with one or more animal oils were explored by using four pairs of species-specific primers for polymerase chain reaction to identify and detect edible oil combined with animal oils. The specificities of the four pairs of species-specific primers were verified using DNA extracted from pig, bovine, sheep, and chicken. Oil from a single animal (pig, bovine, sheep, or chicken oil) was mixed with edible oils in different proportions, the DNA in the oil was extracted using a silica spin column, and a detection level of 0.1% (m/m) single animal-derived component mixed in edible oil was used. Next, the edible oil was combined with two, three, or four types of animal oils and PCR amplification was performed after DNA extraction. The genetic information of each animal was analyzed. Finally, pig-, bovine-, sheep-, and chicken-derived components were identified and detected in the edible oil combined with one or more animal samples, using selected species-specific primers. This method can detect edible oil combined with animal-derived components at a level of 0.1% (m/m) and its provides a new reference method for detected edible oil combinations.
