Determination of Glycidyl Esters in Edible Oils by GC-MS
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Abstract:
An improved method for the determination of glycidyl esters (GEs) in edible oils is described here. Gas Chromatography-Mass Spectrometry (GC-MS) reveled that, after being derivatized with phenylboronic acid, 3-MCPD was successfully released by transesterification with sodium methoxide. For GC-MS analysis, a TG-5MS capillary column (0.25 mm × 30 m × 0.25 μm) was used with a gradient temperature program. The injection and transfer line temperature was 280 ℃ using pulsed splitless mode，and ultrapure grade helium with a flow of 1.18 mL/min as the carrier gas. Electron impact ionization mode was used with an ionization energy of 70 eV. The interface temperature was 280 ℃, with an 8 min solvent delay. The MS was operated in elected ion mode. The limit of detection was 0.010 mg/kg and the recovery rate was 87.64-98.99% with a relative standard deviation of 2.16-5.87%. The content of GEs in several edible vegetable oils was detected to verify this method. Additionally, the effects of oil and fat refining processes on GEs have been studied. The results showed that this method of analysis is both qualitatively and quantitatively accurate，with high sensitivity and good reproducibility. Therefore, this method can meet the demands of the detection and analysis of GEs in edible oils. Among edible vegetable oils, palm oil had a relative high content of GEs. Additionally, analysis of the oil refining process revealed that deodorization was the key process in which GEs were generated.
