Molecular Mechanism Underlying the Amelioration of Dextran Sulphate Sodium (DSS)-induced Colitis in Mice by Oryzanol
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Abstract:
Oryzanol is one of the important active substances in rice bran. A mouse model of ulcerative colitis was established by feeding C57BL/6 mice continuously with 1.0% sulphate sodium D (DSS), and the anti-inflammatory effect of oryzanol and its molecular mechanism were evaluated. The results showed that the symptoms of DSS-induced colitis in the mice from oryzanol group were significantly alleviated. The thickness of bowel wall, colon length, colon weight, inflammatory cell infiltration, disease activity index (DAI) and other important pathological indicators were improved significantly. Oryzanol could also significantly improve important biochemical indicators of inflammatory injury in colonic tissues, such as myeloperoxidase (MPO) and malondialdehyde (MDA) contents, and reduce the plasma nitrite content. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis found that the mRNA expression levels of inflammatory factors, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-1, and IL-6 were significantly reduced in colonic tissues. Western blotting further confirmed that oryzanol suppressed the protein expression of inflammatory factors in colonic tissues. It was concluded that oryzanol had an inhibitory effect on DSS-induced colitis in mice and its molecular mechanism may involve the down-regulation of the expression of inflammatory factors.
