Effect of Culture Media Containing Different Grape Seeds on Phenolic Compounds and the Antioxidant Activity of Pleurotus Ostreatus
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Abstract:
Grape seeds are the major component of mushroom culture media. This study aimed to examine the effects of different grape seeds on the antioxidant activities of Pleurotus ostreatus polyphenols. Four different assays were employed to measure antioxidant activity, including the Folin–Ciocalteu assay, flavonoid content determination assay, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging activity assay, and the ferric reducing ability of plasma assay. Polyphenol content was assessed using high performance liquid chromatography-mass spectrometry (HPLC-MS). Comparison of grape seeds from Cabernet Sauvignon and Longyan species, and different mass fractions (39% and 78%), showed that 78% of Cabernet Sauvignon grape seeds had positive effects on the antioxidant ability of mushroom (69.4% as determined by the ABTS radical scavenging assay) and 10 kinds of polyphenols were identified. Grape seeds can be used for cultivation of mushrooms.