Simultaneous Determination of Sugar, Organic Acids, and Vitamin C in Agricultural Products by Ion-Exclusion Chromatography with Refractive Index and Diode Array Detectors
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Abstract:
A rapid method to determine sugar, organic acids, and vitamin C content in agricultural products was developed by using ion-exclusion liquid chromatography coupled with a diode array detector and a refractive index detector. Tomato and spinach were used as the test samples, which were prepared by homogeneous extraction with 0.05% sulfuric acid for two minutes. The efficiencies of different extracting agents and extraction modes were compared, and the effects of different concentrations of sulfuric acid in the mobile phase on the separation of organic acids were investigated. The results showed that the homogeneous extraction method was both effective and rapid. The ion-exclusion liquid chromatography method demonstrated high accuracy (relative standard deviation: 0.62~1.63%) and good repeatability (coefficient of variation: 0.77~8.54%). The recoveries were in the range of 87.8~109.8% and the measured results were significantly better than those obtained using the national standard method (GB/T).
