Screening of Amine Oxidase-producing Strains from Lactic Acid Bacteria
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Abstract:
In order to obtain starters that can degrade biogenic amines for meat products, 63 strains of lactic acid bacteria previously screened in the laboratory were used as study objects, and the biogenic amine oxidase-producing strains were screened using a color development method on a double-layered plate, the oxidase test, and high-performance liquid chromatography. The bacteriostatic, salt tolerance, and nitrite tolerance tests were conducted for isolated strains. The results showed that the four strains, including one Pediococcus acidilactici strain, one Pediococcus pentosaceus strain, and two Lactobacillus plantarum strains, screened in this experiment, were lactic acid bacteria showing a strong capacity to oxidize biogenic amines. Among the strains, P. acidilactici exhibited the highest biogenic amine degradation, with degradation rates for phenylethylamine, tryptamine, histamine, putrescine, cadaverine, tyramine, spermidine, and spermine of 56.7%, 51.40%, 40.86%, 28.12%, 18.92%, 24.59%, 18.20, and 9.68%, respectively. The results from the bacteriostatic, salt tolerance, and nitrite tolerance tests suggested that P. acidilactici inhibited Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus, with a salt tolerance greater than 6% and a nitrite tolerance greater than 0.15 g/kg; the optimum temperature and pH were 37 ℃ and 6.5, respectively.
