Construction of Engineering Escherichia coli for p-Coumaric Acid Bioproduction
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Abstract:
p-Coumaric acid is a bioactive phenolic compound that can prevent cardiovascular disease, with antioxidant, antibacterial, and anti-inflammatory properties. It is also a precursor of high-value phenylpropanes nutraceuticals (such as resveratrol). A new method for p-coumaric acid bioproduction was developed in this study to increase production levels to meet the growing demand for this compound. The constitutive expression vector containing tyrosine ammonia lyase gene from Rhodotorula glutinis (RgTAL) was transformed into host strain, Escherichia coli ATCC31884. The recombinant strain was confirmed by polymerase chain reaction (PCR). Fermentation broth using the recombinant strain was analyzed by high performance liquid chromatography (HPLC) and p-coumaric acid bioproduction by the engineered E. coli strain was confirmed. Optimal concentration of L-tyrosine substrate to be added and fermentation time were identified; highest yield of p-coumaric acid (161.23 mg/L) was achieved with 0.5 mM L-tyrosine and 36 h fermentation. The results indicated that Rgtal gene was successfully expressed in the recombinant engineered E. coli strain, which was capable of p-coumaric acid biosynthesis via its own metabolism, using exogenously supplemented L-tyrosine.
