Correlation between Masked T-2 Toxin Residues and Changes in Fat-Soluble Components in Muscles of Litopenaeus vannamei
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Abstract:
This study explored the content of masked T-2 toxin (mT-2) residues and the effect of this toxin on fat-soluble components in T-2 toxin-exposed muscle tissues of shrimp (Litopenaeus vannamei). L. vannamei muscle tissues from the groups treated with different doses of T-2 toxin (0, 0.5, 1.2, 2.4, 4.8, and 12.2 mg/kg feed) after 20 days of cumulative exposure. Content of the T-2 toxin in the muscle tissues before and after trifluoroacetic acid (TFA) hydrolysis was determined by performing liquid chromatography–tandem mass spectrometry; content of mT-2 was expressed by the increment of T-2 toxin. Crude fat content was determined by using Soxhlet method, and vitamin A (VA), vitamin D3 (VD3), and vitamin E (VE) content was determined by performing high-performance liquid chromatography. Free T-2 toxin was not detected in shrimp muscle tissues before TFA hydrolysis but was detected after TFA hydrolysis. Moreover, its content was positively correlated with the dose of T-2 toxin administered. Different doses of the T-2 toxin exerted significantly different effects on the content of fat-soluble components. Treatment of shrimps with a high dose of the T-2 toxin significantly decreased crude fat, VA, and VD3 content (p< 0.05) but fluctuated VE content. Treatment of shrimps with a low dose of the T-2 toxin significantly increased crude fat, VD3, and VE content (p < 0.05), indicating a low-dose stimulatory effect. Crude fat content was negatively correlated and VA and mT-2 toxin content was positively correlated with the existing form of the T-2 toxin. These results can be used as a reference to determine the indicators of mT-2 toxicity.
