Hemostatic Activity of Pollock Gelatin and the Underlying Mechanism
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Abstract:
To improve the utilization of aquatic byproducts and provide a basis for the development of gelatin as a hemostatic product, the relationship of the molecular weight of gelatin to its hemostatic activities and mechanisms was studied. Pollock skin gelatin was extracted using the hot water method at 65 ℃, and its molecular weight distribution was measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). High, medium, and low molecular weight gelatin samples were obtained though ultrafiltration using 100 ku and 50 ku membranes. Rat tail cutting, femoral artery cutting, and liver cutting experiments showed that high molecular weight gelatin resulted in the shortest bleeding times, which were 4.54 min, 2.30 min and 1.91 min, respectively. Pollock gelatin exhibited hemostatic activity as compared to the control group. Bleeding time and the amount of bleeding declined with increasing gelatin molecular weight. Thus, gelatin with a high molecular weight exhibited a relatively good hemostatic effect. An activated partial thromboplastin time (APTT) test indicated that gelatin significantly reduced APTT and affected the endogenous coagulation pathways. It also promoted platelet aggregation and increased the release of platelet active factors platelet factor 4 (PF4), P-selectin, and thromboxane B2 (TXB2), thereby shortening bleeding time and showing a hemostatic effect.