Isolation and Identification of an L-tyrosine-producing Endophyte from Zanthoxylum bungeanum
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Abstract:
Six endophytic strains were isolated from the fruits of Zanthoxylum Bungeanum by grinding, and these included four strains of bacteria and two strains of fungi. The endophytic bacteria, Z2, was used as the research object in the present study, and its morphological, physiological, biochemical, and molecular biological characteristics were investigated. It was found that the strain Z2 was Gram-positive without spores and flagella, and was observed as a short rod of about 1.5~2.5 μm in length and 0.6~0.7 μm in width. According to the results on the physiological and biochemical characteristics, 16S rDNA gene sequence, and the phylogenetic tree, Z2 was identified as Brevibacterium frigoritolerans. During comparative analysis of the broth fermentated with Z2 and the broth without fermentation using high-performance liquid chromatography (HPLC), a new substance was found in the fermentation broth. This new substance was further purified by Sephadex LH-20 column chromatography and by semipreparative HPLC. Based on of the data from electrospray ionisation tandem mass spectrometry and nuclear magnetic resonance imaging, this substance was identified as L-tyrosine and its content was 12.90 mg/L in the fermented broth.
