Effect of Sucrose Treatment on Flavonoid Content and Antioxidant Activity of Sedum aizoon Leaves
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Abstract:
Young twigs from Sedum aizoon L. were incubated in media containing various concentrations of sucrose (5, 10, 20, 30, and 40 mmol/L). The medium containing 20 mmol/L sucrose exhibited a relatively good flavonoid accumulation during the incubation, with the flavonoid content (wet weight) of Sedum aizoon L. cultured in this medium being 1.58 times greater than that of the control group. This medium was selected to study the antioxidant capacity of Sedum aizoon L. Sucrose treatment significantly increased the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and total phenolic content. The sucrose-treated group had higher activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and peroxidase (POD) than the control group due to the impact of exogenous sucrose. In addition, the phenylalanine ammonia-lyase (PAL) activity for the sucrose-treated group was higher than that of the control group. These results indicate that sucrose treatment enhances flavonoid content and antioxidant activity. These results provide a new approach and a theoretical basis for flavonoid accumulation using Sedum aizoon L.
