Stability of Antioxidant Peptides Extracted from Pseudosciaena crocea Viscera
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Abstract:
The stability of the antioxidant activity of antioxidant peptides extracted from Pseudosciaena crocea viscera were studied under different conditions. Peptides with strong antioxidant activity were selected by size exclusion chromatography (SEC), and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH?) radical scavenging rate and Fe2+ chelating capacity as indexes. Under conditions of neutral pH, room temperature, low NaCl concentration, and appropriate amounts of K+,Ca2+, and Al3+, the antioxidant activity of the antioxidant peptides from Pseudosciaena crocea viscera was generally stable, and DPPH? radical scavenging rate and Fe2+ chelating capacity were 88.5% and 66.7%, respectively. With high NaCl concentration, high temperature, additions of K+, Ca2+, and Al3+, and long-duration ultraviolet irradiation, the antioxidant activity of the antioxidant peptides from Pseudosciaena crocea viscera was reduced by 30%. Furthermore, at acidic pH and additions of glucose, fructose, and lactose at 70 ℃, the DPPH? radical scavenging activity of the antioxidant peptides from Pseudosciaena crocea viscera was increased to approximately 90%; while alkaline conditions were conductive for the Fe2+ chelating capacity, indicating that treatments have different impacts on different antioxidant indicators. In addition, pepsin digestion treatment had no significant impact on the antioxidant activity of antioxidant peptides from Pseudosciaena crocea viscera, and further chymotrypsin digestion significantly decreased the antioxidant activity stability of the peptides. Therefore, appropriate processing and storage are critical for maintenance of the stability of the antioxidant peptides extracted from Pseudosciaena crocea viscera.
