Extracellular Metabolic Profiling of Common Food-borne Pathogens
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Abstract:
This study aims to determine the characteristic metabolites of common food-borne pathogens and develop fast and efficient techniques for food safety monitoring. In this study, the zymotic fluid of food-borne pathogens was freeze-dried and derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), followed by analysis using gas chromatography mass spectrometry (GC-MS). An NIST11 database search and classification were also performed, and the acquired data were analyzed using heatmapping and principal component analysis (PCA). The results showed that there were a large number of substances, such as organic acids, alcohols, and amines that were formed in the culture broth. Significant differences were observed between different bacterial species based on the types and relative metabolic contents. Many specific metabolites for each bacterial species were found, some of twhich have never been reported in other strains. The extracellular metabolic profiles of all bacteria were analyzed by heatmapping and PCA, and the results showed that the strains could be differentiated at 24 h; the differentiation effect of PCA was significantly enhanced after the removal of amino acid and sugars, and the optimal effect was found at 24 h. Our results show that extracellular metabolic profile analysis can be used to determine the biomarkers of food-borne pathogenic bacteria and distinguish between bacterial species. Further, some of the characteristic metabolites may be potential biomarkers of common food-borne pathogens.
