Protective Effect of Wheat Germ Peptide on Cultured SH-SY5Y Human Neuroblastoma Cell Line in an in vitro Cell Injury Model
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The protective effects of wheat germ peptide (RVF) on the SH-SY5Y human neuroblastoma cell line were investigated in this study. Starvation- and hydrogen peroxide (H2O2)-induced injury models were established by in vitro cell culturing of an SH-SY5Y human neuroblastoma cell line. The SH-SY5Y cells were treated with 40 RVFs at different concentrations and incubation times. The results showed significant nutritional functions only in the group pre-incubated with 200 μmol/L RVF for 12 h in a no-serum starvation-induced injury model and the group treated with 500 μmol/L RVF for 24 h in the 1% serum starvation-induced injury model, and no dose- or time-dependent relationships were found . In the H2O2-induced injury model, treatment with 200 μmol/L RVF increased cell survival rates by 25.05 and 21.90% in the methyl-tetrazolium (MTT) and sulforhodamine B (SRB) assays, respectively, in a time- and dose-dependent manner. The cell morphological analysis indicated that after RVF treatment, the SH-SY5Y cells gradually resumed a spindle or polygonal shape from a wrinkled, shrunken, and elongated shape, with enhanced cell adhesion. In the two injury models investigated in this study, RVF only showed a significant protective effect in the H2O2-induced injury model.
