Isolation and Identification of Active Ingredients in Rubus corchorifolia L.f. Leaves and Their Bioactivities
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Abstract:
The ethanol extract of Rubus corchorifolia leaves was used in this study, and its active compounds were isolated and purified using solvent extraction, silica gel column chromatography, and crystallization methods. The structures of the isolated monomeric compounds were identified using nuclear magnetic resonance (NMR), mass spectrometry (MS), infrared (IR) spectroscopy, and comparisons with literature data. The antibacterial and antitumor activities of the monomeric compounds were studied by resazurin microdilution (96-well plate) assay and MTT assay. The two compounds isolated and purified from Rubus corchorifolia leaves were identified as 2α,3β,23α-trihydroxy-12-en-28-ursolic acid (1) and flavonoid compound kaempferol-3-O-β-D-6′′-p-hydroxycoumaroyl-glucoside (2). The minimum inhibitory concentrations (MICs) of compound 1/compound 2 on Escherichia coli, Shigella dysenteriae, Staphylococcus aureus, and Salmonella were 15.6 μg/mL/31.25 μg/mL, 15.6 μg/mL/62.5 μg/mL, 31.25 μg/mL/62.5 μg/mL, and 15.6 μg/mL/62.5 μg/mL, respectively. The half inhibitory concentration (IC50) of compound 1 on HepG2, MCF-7, and OVCAR3 cells was 34.2 μM, 60.43 μM, and 62.3 μM, respectively. Compound 1 showed good antibacterial and antitumor activities, while the inhibitory effects of compound 2 on HepG2, MCF-7, and OVCAR3 cell lines were not significant.
