Quorum-sensing Phenomenon in Pseudomonas fluorescens Isolated from Turbot and Spoilage Characteristics Using Different Carbon Sources
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Pseudomonas fluorescens isolated from turbot was used as a test strain. Quorum-sensing signal molecules were detected by a parallel streak method using Chromobacterium violaceum CVO26 strain. The bacteria were cultured in AB medium supplemented with different carbon sources (glucose, sucrose, fructose, xylose, lactose, or maltose), and biofilm formation, and siderophore and extracellular protease production was measured. Simultaneously, N-acylhomoserine lactones (AHLs), a class of external quorum-sensing compound, were added to the culture in order to determine the relationship between AHLs and spoilage factors. Quorum-sensing phenomenon was observed in the tested strain culture, and the production of spoilage factors significantly differed between different carbon sources used. The addition of carbon sources promoted biofilm formation, but it had no significant influence on the production of extracellular protease. The biofilm formation was relatively high when glucose and maltose were used as the carbon source, and a relatively high rate of siderophore production was observed when sucrose and lactose were used. The biofilm formation and siderophore and extracellular protease production were significantly enhanced when the external quorum-sensing signal molecules were added. Therefore, the biofilm formation and siderophore and extracellular protease production are related to AHLs, and quorum-sensing phenomenon might control the effects of spoilage factors, and play a role in the spoilage of aquatic products.
