Evaluation of Antioxidant Activity of Polypeptides from Abalone Viscera Using In vitro Chemistry and Cell Culture Model
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The scavenging activity of abalone visceral polypeptide (AVPP) against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH?), hydroxyl radical (?OH), and superoxide anion radical (O2-?) was determined in this study. The results showed that AVPP exhibited certain free radical scavenging activity against DPPH?, ?OH, and O2-?-, with IC50 values of 0.87, 7.53, and 19.41 mg/mL, respectively. An oxidative stress injury model was established by human hepatoma cells (HepG2) treatedwith H2O2. HepG2 cells were cultured in vitro and divided into five groups: control, model, low-dose AVPP (1.6 mg/mL), medium-dose AVPP (3.2 mg/mL), and high-dose AVPP (6.4 mg/mL). Cell viability, total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) levels in each group were measured. The results showed that the AVPP significantly increased HepG2 cell viability at doses of 1.6 mg/mL, 3.2 mg/mL, and 6.4 mg/mL. Additionally, the AVPP increased T-AOC and SOD activity and markedly reduced the MDA levels in a positive dose-response pattern. Thus, the results indicate that AVPP possesses excellent antioxidant capability.
