Isolation of Polysaccharides from Perna viridis and Their Antioxidant Activities in High-fat Diet-fed Mice
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Abstract:
Previously, the Perna viridis polysaccharides (PVPs) were demonstrated to have a remarkable hypolipidemic effect in a mouse model of hyperlipidemia. Herein, the PVPs were purified and analyzed, and their antioxidant effects were evaluated in a mouse model of hyperlipidemia. Two main fractions P1 and P2 were separated from PVPs by DEAE-52 and Sephadex G-200 column chromatography. Chemical analysis revealed that P1 and P2 were acidic polysaccharides with sulfate groups, and their relative molecular weights (MWs) were 6.89 × 105 Da and 2.87 × 105 Da, respectively. The content ratios of glucosamine, uronic acid, and galactosamine for P1 and P2 were 3.7:2.6:1 and 2.8:1.6:1, respectively. High-fat diet-fed mice (a diet supplemented with 2% cholesterol) were intragastrically administered low dose [50mg/(kg?d)], medium dose [100mg/(kg?d)], and high dose of PVPs [200mg/(kg?d)]. The results showed that the medium dose and high dose of PVPs could significantly enhance the total antioxidant capacity (T-AOC), as well as glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and reduce the malondialdehyde (MDA) content in serum, the liver, and the brain of the hyperlipidemic mouse model (p < 0.01) or (p < 0.05). The results demonstrate that PVP can exert a hypolipidemic effect by activating the activities of antioxidase in vivo and by reducing the level of peroxidation in hyperlipidemic mice.
