Antioxidant and Antiproliferative Activities of Polyphenols in Morchella umbrina Boud on the HepG2 Cell Model
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Abstract:
The in vivo antioxidant capacity of free and bound phenols in Morchella umbrina Boud. and their toxic and antiproliferative effects on HepG2 cells were investigated in this study. The polyphenolic components in Morchella umbrina Boud were identified by high performance liquid chromatography (HPLC), in addition to measuring the oxygen radical absorption capacity (ORAC). The human liver cancer cell line HepG2 was used as a model to measure cellular antioxidant activity (CAA) and antiproliferative activity (EC50 value). The results showed that the free phenol and bound phenol content of Morchella umbrina Boud was (4.27 ± 0.07) mg GAE/g DW and (0.14 ± 0.01) mg GAE/g DW, respectively. The ORAC values of free phenol and bound phenols were (4624.52 ± 400) and (429.41 ± 60) μmol TE/100 g DW, respectively. The corresponding CAA values with PBS washing were 15.33 ± 0.60 and 0.37 ± 0.01 μmol QE/100 g DW, respectively. The corresponding CAA values without PBS washing were 51.84 ± 0.83 and 1.27 ± 0.04 μmol QE/100 g DW, respectively. HepG2 cell proliferation was inhibited at a ratio of 77.28% and 66.31% at 125 μg/mL for free phenol and 70 μg/mL for bound phenol, respectively. In addition, within the concentration range of the experiment, the polyphenols from M. umbrina Boud generally showed no cytotoxicity to HepG2 cells. These results indicate that the polyphenols in M. umbrina Boud have certain antioxidant and antiproliferative effects. Thus, M. umbrina Boud could be used as a raw material to develop related types of functional foods. This study provides a theoretical basis for further utilization of M. umbrina Boud.
