A Method for Detection of Zeaxanthin and Its Stereoisomers
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Abstract:
In order to establish a method to accurately and effectively detect zeaxanthin and its stereoisomers, the zeaxanthin products formed by iodine-catalyzed photoisomerization were separated using different mobile phases, ratios of the mobile phase, and elution times. The qualitative and quantitative analyses of zeaxanthin and its stereoisomers were conducted using spectroscopy, chromatography and mass spectrometry. The results indicated that the optimum conditions for the detection of zeaxanthin and its stereoisomers were as follows: mobile phase A: methanol, methyl tert-butyl ether (MTBE), and water (ratio, 80/15/5); mobile phase B: methanol and MTBE (ratio, 1/10); elution time: 24 minutes. In the zeaxanthin products from the iodine-catalyzed photoisomerization, 15,15′-, 13,13′-, and 9,9′-mono-cis-zeaxanthin isomers were identified by comparing the maximum absorption wavelength for each peak, Q value, and ion fragments in mass spectra with those in related reports. Peak area and injection volume of all-trans-zeaxanthin showed a good linear relationship in the range of 5–250 ng. The proposed method showed advantages including good reproducibility, high accuracy, and rapid detection, which is suitable for the detection of zeaxanthin and its stereoisomers.
