Preparation and Application of a Molecularly Imprinted Solid-phase Extraction Membrane with High Selectivity to Diniconazole
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Abstract:
Molecularly-imprinted, solid-phase extraction membrane with high selectivity to diniconazole was prepared by in situ polymerization using diniconazole as the template molecule and acrylamide (AM) as the functional monomer. AM was optimized and the binding ability of different functional monomers with the template molecule was investigated by ultraviolet (UV) spectroscopy. This property was also used to select porogens for preparation of molecularly imprinted membrane and the influence of imprinting time on liquid permeability. A method based on molecularly imprinted, solid-phase membrane, high-performance liquid chromatographic was developed for the determination of diniconazole residues in grains. The results showed that compared with the functional monomer α-methacrylic acid (MAA), AM showed a stronger binding capacity to diniconazole. When the molecularly-imprinted diniconazole membrane was prepared using acetonitrile as the porogen, the once-imprinted membrane was stable with a good liquid permeability. A good linear range of diniconazole was obtained in the range of 0.5~15 μg/mL (r = 0.9987). The average recoveries ranged from 80.34% to 87.03%, and the detection limit of diniconazole was 2.0 μg/g. Thus, the method developed in this study is highly selective, sensitive, and reliable, which can be applied for determination of diniconazole residues in grain and other complex matrices.
