Application of De novo Sequencing in the Whole Genomic Study of Beer-spoilage Lactobacilli
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Abstract:
The whole genome of a typical hard-to-culture beer-spoilage Lactobacilli strain which had never been sequenced was analysized. The Lactobacilli strain was isolated from beer and its genomic DNA was extracted and purified. A high-quality sequencing library was constructed and the whole genome sequencing was then performed using De novo system. The sequences were further analyzed and the predicted genes were obtained, including GO gene function, COG gene function and KEGG pathway. In the whole genome sequences of this Lactobacilli strain, 1,824 genes were predicted and 545, 1,303, and 120 of them had GO, COG, and KEGG annotation, respectively. This study may provide more references for the study of functional gene and bioinformatics analysis of the beer-spoilage lactobacilli strains .
