Effect of Yunnan Large-Leaf Camellia Sinensis Extract on Growth and Aflatoxin Production of Aspergillus flavus
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Abstract:
Various microbes are involved in the fermentation of pu-erh tea. , among themAspergillus niger plays a crucial role in this process. Therefore, some consumers worry about the contamination of pu-erh tea with aflatoxins (AFs). In this study, the effect of Yunnan large-leaf Camellia sinensis extract on the growth of A. flavus AS3.4408 was studied by measuring the colony diameter, spore germination, and mycelial dry weight. The effect of Yunnan large-leaf Camellia sinensis extract on the biosynthesis of AFs was studied using ultraviolet fluorescence analysis and HPLC. Tea containing Yunnan large-leaf Camellia sinensis extract was inoculated with A. flavus AS3.4408 and the AF content in the tea was measured in order to evaluate the safety of pu-erh tea. The results showed that the growth and AF production of A. flavus AS3.4408 were significantly inhibited by the Yunnan large-leaf Camellia sinensis extract in a dose-dependent manner. After A. flavus AS3.4408 was inoculated into the tea containing the Yunnan large-leaf Camellia sinensis extract, the strain grew well. However, the AF B1, B2, G1 and G2 were not detected by HPLC, indicating that AF biosynthesis was inhibited by some components of Yunnan large-leaf Camellia sinensis.
